Characterization of the mechanism of action of Escherichia coli heat-stable enterotoxin
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چکیده
منابع مشابه
Mechanism of action of Escherichia coli heat stable enterotoxin in a human colonic cell line.
Escherichia coli heat stable enterotoxin (STa) caused Cl- secretion across T84 cell monolayers in a dose-dependent manner only when applied to the apical membrane surface and not when applied to the basolateral surface. Measurement of cAMP, cGMP, and free cytosolic Ca2+ in response to STa suggested that cGMP alone mediated the Cl- secretory response. Studies utilizing blockers of the Na+,K+-ATP...
متن کاملThe mechanism of action of Escherichia coli heat-stable toxin.
Holmgren, J. (1981) Nature (London) 292, 413417 Hughes, J . M., Murad, F., Chang, B. & Guerrant, R. L. (1978) Nature (London) 271, 755-756 Hughes, S., Warhurst, G., Turnberg, L. A,, Higgs, N. B., Giugliano, L. G. & Drasar, B. S. (1983) Gut 24 (2), 94-98 James, C., Dibley, M., Burke, V., Robinson, J. & Gracey, M. (1982) Clin. Expt. Immunol. 47, 3 4 4 2 Leppla, S. H. (1982) Proc. Natl Acad. Sci. ...
متن کاملProperties of synthetically produced Escherichia coli heat-stable enterotoxin.
The properties of a synthetically produced peptide composed of the same primary structure of 18 amino acids described for human Escherichia coli heat-stable enterotoxin were compared with those of purified heat-stable toxin obtained by bacterial growth. The dosage required to evoke fluid secretion in the suckling mouse and rat ligated ileal loop assays was the same for both toxins. The antigeni...
متن کاملProtection in rats immunized with Escherichia coli heat-stable enterotoxin.
Rats immunized with a semipurified preparation of the Escherichia coli heat-stable (ST) enterotoxin conjugated with a protein carrier were protected against challenge with semipurified or purified ST and viable organisms of multiple heterologous serotypes that produce only ST (LT-/ST+), but they were not protected against heal-labile (LT) toxin or viable strains which produce LT either alone (L...
متن کاملEnzyme-linked immunosorbent assay for Escherichia coli heat-stable enterotoxin.
The sensitivity of an enzyme-linked immunosorbent assay (ELISA) to detect pure native Escherichia coli heat-stable toxin (ST) and to identify ST-producing strains among clinical isolates was determined. Two synthetically produced ST preparations were used to raise hyperimmune antisera in rabbits and goats: ST(S), which has the same antigenicity as native ST; and ST(C), which is 15-fold more imm...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1984
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.44.2.493-501.1984